Reporter

Part:BBa_K770001:Design

Designed by: Michel Saremi   Group: iGEM12_Copenhagen   (2012-09-22)


LuxCDABE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1311
    Illegal SapI site found at 3450


Design Notes

Contained an illegal Xbal site that had to be removed, we did so by introducing a USER-site over the restriction site containing a point mutation, this devided our LuxCDABE in two pieces of aproxymately 2 kbp and 4 kbp, which we assembled by USER-cloning with a pSB1C3 backbone, this procedure has flanked our LuxCDABE with USER-scars located between the cassette itself and our biobrick required prefix and suffix. This should not affect the transcription of the cassette itself. The sequence of the scars and their sequence are given below. Prefix-AGTGCGAT-LuxCDABE-ACTTGCGT-Suffix



Source

Plasmid DNA


References